Isolation and Characterization of Biosurfactant-producing Cytobacillus oceanisediminis MS-05A
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Abstract
Biosurfactants are amphiphilic molecules that can reduce the surface tension of liquid. They are non-toxic, biodegradable and eco-friendly. Chemically synthesized surfactants are toxic to human health and causes skin diseases. So, there is an extensive need of non toxic and Biodegradable biosurfactant. Attempt has been made for screening and isolation of biosurfactant producing bacteria from Ratnagiri sea side soil. The isolates from soil samples were screened by using hemolytic activity, drop collapse test, tilted glass test and oil displacement activity. The potent isolate MS-05A was characterised at molecular level. It showed 99.64% similarity with Cytobacillus oceanisediminis H2 (GQ292772). The growth of isolate MS-05A was optimized in Zobell marine broth for different environmental and nutritional parameters like salt, pH, temperature, agitation and sugar concentration. Optimal conditions were used for growth, emulsification activity and extraction of biosurfactant. The crude biosurfactant of MS-05A was characterized with thin layer chromatography (TLC) and fourier transform infrared spectroscopy (FTIR). Washing performance test and antimicrobial activity of crude biosurfactant were tested. The isolated MS-05A showed optimal growth in Zobell marine broth containing 3% sucrose, 5% salt, at 35°C,120 RPM agitation, pH 7 for 4 days incubation. The cell free supernatant of isolate showed emulsification activity 55% for sunflower oil. The crude biosurfactant of MS-05A showed good washing performance and antimicrobial activity. It may be used as a biocontrol agents in agriculture.
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